RNA

Part:BBa_K2381024:Design

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-11-01)


J23119-gRNA (tightly target at oriC to inhibit cell replication)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no.


Source

It is designed from sequence originating from Streptococcus pyogenes.

References